Lumbar puncture

A lumbar puncture is a diagnostic and therapeutic procedure in which a spinal needle is passed into the subarachnoid space. It enables drainage or collection of cerebrospinal fluid (CSF) as well as administration of intrathecal medications. CSF analysis may aid in the diagnosis of meningitis, multiple sclerosis, intracranial hemorrhage, or meningeal carcinomatosis. In addition, drainage of CSF can lead to symptomatic improvement in patients with idiopathic intracranial hypertension and normal pressure hydrocephalus. There are no absolute contraindications to a lumbar puncture, although increased intracranial pressure, bleeding disorders, and spinal abscesses all increase the risk of complications. A common and unpleasant, albeit harmless, complication of lumbar puncture is a post-lumbar puncture headache.

• Diagnosis of diseases with altered CSF composition
  • Central nervous system infections (bacterial, viral, mycobacterial)
  • Central nervous system malignancies and paraneoplastic syndromes
  • Guillain-Barré syndrome
  • Multiple sclerosis
  • Neuroborreliosis
  • Subarachnoid hemorrhage (SAH)
• Intrathecal administration of pharmaceuticals
  • Antibiotics
  • Chemotherapy
  • Anesthesia
  • Contrast
• CSF pressure measurement
  • Idiopathic intracranial hypertension (pseudotumor cerebri)
  • Normal pressure hydrocephalus

Suspected meningitis or SAH in a patient with a negative CT scan is an urgent indication for a lumbar puncture!

References:^[1]

1. Explain the procedure to the patient (i.e., the application, purpose, possible complications).
2. Choose a needle type
   • Pencil point or “atraumatic” needles are associated with lower rates of post-lumbar puncture headache, although conventional “cutting” needles may also be used.
   • 20- or 22-gauge spinal needles are typically used.
3. Determine the level of entry while patient is upright.
   • Identify L4, which is typically located at the level of the iliac crest
   • The spinal needle may be inserted either above (L3-L4 interspace) or below (L4-L5 interspace).
4. Position the patient in the lateral recumbent fetal position.
   • The lower lumbar spine should be in flexion.
   • Alternatively, the patient may be positioned sitting upright or lying prone.
5. Disinfect the skin over the desired entry point with alcohol and chlorhexidine or povidone-iodine.
6. Wear sterile gloves and place a sterile drape over the patient with an opening over the desired entry point.
7. Apply local anesthesia to the desired entry point.
8. Angle spinal needle slightly towards the umbilicus and advance slowly (the bevel should point towards the patient's side).
9. Initial resistance followed by a noticeable loss of resistance indicates piercing of the ligamenta flava/dura and entry into the dural sac.
10. Remove stylet after puncture and observe for flow of CSF, which indicates successful entry into the subarachnoid space.
    • If CSF pressure measurement is requested: Place a manometer over the needle hub to measure an opening pressure.
    • If administering pharmaceuticals: Inject the drug (e.g., local anesthetic such as bupivacaine → spinal anesthesia).
    • In diagnostic or therapeutic lumbar puncture: Serially collect CSF without active aspiration (up to 40 mL may be collected).
11. After completion of puncture: Insert the stylet back into the puncture needle. , withdraw the needle, apply a sterile swab, and compress the puncture site.
12. Recommend bedrest for 1–2 hours and sufficient fluid intake.
13. Document the lumbar puncture in the patient's file. and check labeling on the tubes.
14. Transport/storage: If possible, immediate transport of the samples to the laboratory should be arranged. Otherwise, they should be placed in refrigerated storage (4°C).

References:^[1][2]

Cerebrospinal fluid analysis
	Opening pressures (see also elevated intracranial pressure and brain herniation)	Appearance	Cell type (number/μL)		Lactate	Protein	Glucose
Normal	≤15 mm Hg	Colorless and transparent	Acellular (< 5 WBCs and < 5 RBCs)		1.2–2.1 mmol/L	15–45 mg/100 mL	40–75 mg/100 mL (60% of serum levels)
Multiple sclerosis	Normal	Colorless and transparent	↑ WBCs (< 50)		Normal	Normal to ↑	Normal
Guillain-Barré syndrome	Normal	Colorless and transparent	↑ WBCS (< 10)		Normal	↑↑	Normal
Subarachnoid hemorrhage, stroke	Normal or ↑	Bloody or xanthochromic (i.e., pink or yellow if hemorrhage > 6 h prior to sampling)	↑ RBCs, ↑ WBCs		Normal	↑ (gamma globulin)	Normal
Brain tumors	Normal or ↑	Colorless and transparent	Drop metastases		Normal	↑	↓
Pseudotumor cerebri (idiopathic intracranial hypertension)	↑↑	Colorless and transparent	Acellular		Normal	Normal	Normal
Meningitis	↑–↑↑	Colorless and transparent or cloudy	Pleocytosis, lymphocytosis, or granulocytosis See cerebrospinal fluid analysis in meningitis		Variable	Normal or ↑	Normal or ↓

• Gram stain and culture to differentiate pathogens (see diagnosis of meningitis)
• Detection of special markers, e.g., tumor markers, tau protein, PCR, or serology

Disruption of the blood-brain barrier (i.e., infections, autoimmune diseases, CNS malignancies) or intrathecal production of IgG (i.e, multiple sclerosis, CNS infections such as Lyme disease) → increased immunoglobulins (oligoclonal bands) → increased CSF protein!

References:^[3][4][5]