Carbohydrates

Overview

Definitions

Lactase is the only enzyme in the human body that can cleave β-glucosidic bonds, but only those of the disaccharide lactose. There are no enzymes in the digestive tract that can cleave β-glycosidic bonds of polysaccharides. Cellulose therefore remains undigested in the intestine and is referred to as fiber!

Important disaccharides

Name Composed of

Sucrose

Common table sugar

  • Glucose + fructose
Maltose
  • Glucose + glucose

Lactose

Milk sugar

  • Glucose + galactose

Digestion and reabsorption

Carbohydrates in food

Digestion

Enzyme Site Chemical reaction
α-Amylase Polysaccharidesmaltose, isomaltose, maltotriose, other oligosaccharides
Lactase Lactose → galactose + glucose
Sucrase-isomaltase Maltose, isomaltose, maltotriose, saccharose → glucose + fructose
Maltase-glucoamylase Polysaccharides, oligosaccharides, disaccharides → glucose

Absorption

Glucose enters intestinal epithelial cells and proximal renal tubular cells via Na+-glucose-cotransporters (SGLT). In all other cells of the body, glucose uptake occurs through specific membranous glucose transporters (GLUT).

  • Intestinal glucose absorption
    • Sodium-dependent glucose cotransporter 1 (SGLT1)
      • Specific transporter on the luminal side of mucosa cells, as well as the proximal straight tubule in the kidney
      • The driving force is a sodium concentration gradient, which is maintained by basal Na+/K+ ATPase by transporting sodium out of the cell (secondary active transport).
      • Sodium moves down its concentration gradient into the cell and takes a glucose molecule with it each time.
      • Also absorbs galactose.
  • Transport into blood: via GLUT2 and circulates unbound in blood
  • Glucose uptake into cells
  • Renal glucose reabsorption
    • Free filtration of glucose by the kidneys
    • Complete reabsorption in the proximal tubules via 2 types of SGLT urine normally glucose free
      • SGLT2: reabsorbs ∼ 98% of urinary glucose in the proximal convoluted tubule (PCT)
        • One molecule of glucose is absorbed together with one molecule of sodium.
      • SGLT1: reabsorbs the remaining glucose (∼ 2%) as well as galactose in the PST
        • One molecule of glucose is absorbed together with two molecules of sodium.
      • Fructose is absorbed via GLUT5 (glucose transporter).
      • Reabsorption also relies on a sodium concentration gradient via Na+/K+ ATPase.

Important glucose transporters

Name Site Special function Insulin-dependent
GLUT1
  • No
GLUT2
  • Transports all monosaccharide from the basolateral membrane of enterocytes into the blood
  • Glucose sensor: The free flow of glucose in and out of pancreatic cells allows measuring of serum glucose levels.
  • Low affinity (KM) for glucose → glucose only diffuses at high concentrations
  • Bidirectional transporter: allows hepatocytes to uptake glucose for glycolysis and release glucose during gluconeogenesis
  • No
GLUT3
  • No
GLUT4
  • Plays a key role in regulating body glucose homeostasis
  • Insulin stimulates incorporation of GLUT4 (stored in vesicles) into plasma cell membranes of cells for controlled glucose uptake and storage
  • Physical exercise also induces the translocation of GLUT4 into the plasma membrane of skeletal muscle (insulin-independent!)
  • Yes
GLUT5
  • Small intestinal enterocytes
  • Spermatocytes
  • Fructose transporter
  • No

Only GLUT4 is insulin-dependent!

Maldigestion and malabsorption

Glucose metabolism

Galactose metabolism

Absorption of galactose

Galactose is part of lactose (found in milk products).

  1. Lactose is cleaved in the small intestine by the lactase.
  2. Freed galactose is absorbed by enterocytes via SGLT1.
  3. Transported into blood via GLUT2
  4. Circulates to the liver for further metabolism

Breakdown of galactose

Galactose metabolism converges with glucose metabolism after some intermediate steps: galactose → galactose-1-phosphateUDP-galactoseUDP-glucoseglucose-1-phosphate (enters glycolysis).

  1. Galactokinase activates galactose: galactose + ATPgalactose-1-P + ADP
  2. Galactose-1-phosphate uridylyltransferase: galactose-1-P + UDP-glucoseUDP-galactose + glucose-1-P (can be fed into glycolysis)
  3. UDP-galactose 4-epimerase: UDP-galactoseUDP-glucose

If an individual is deficient of the enzyme galactose-1-phosphate UDT transferase (classical galactosemia), galactose and lactose (galactose + glucose) have to be removed from the diet!

High blood levels of galactose also result in conversion to the osmotically active galactitol via aldose reductase. In individuals with galactokinase deficiency, excess galactitol forms in the lens of the eye and leads to early-onset cataracts!

Galactose synthesis

Disorders of galactose metabolism

  • Galactosemia

Fructose metabolism

Absorption of fructose

Fructose is part of sucrose (a common table sugar).

  1. Sucrose is cleaved in the small intestine by sucrase-isomaltase.
  2. Freed fructose is absorbed into enterocytes via facilitated diffusion by GLUT5.
  3. Transported into blood via GLUT2
  4. Circulates to the liver for further metabolism

Breakdown of fructose (fructolysis)

Fructose metabolism converges with glucose metabolism after some intermediate steps: fructose + ATPfructose-1-Pglyceraldehyde-3-Pglycolysis

  1. Fructokinase activates fructose: fructose + ATPfructose-1-P + ADP
  2. Aldolase B splits hexose into two trioses: fructose-1-Pdihydroxyacetone-P + glyceraldehyde
  3. Conversion to glyceraldehyde-3-P:
  4. Glyceraldehyde-3-P is fed into glycolysis

If an individual is deficient in the enzyme aldolase B (hereditary fructose intolerance), both fructose and sucrose (glucose + fructose) have to be removed from the diet!

Fructose synthesis

Fructose can be produced from glucose via sorbitol (osmotically active sugar alcohol) without using ATP.

  1. Aldose reductase reduces glucose to sorbitol: glucose + NADPHsorbitol
  2. Sorbitol dehydrogenase oxidates sorbitol to fructose: sorbitol + NAD+ → fructose
    • In liver, ovaries, seminal vesicles
    • In the body, fructose is the primary source of energy for spermatozoa.

Tissues that do not have sorbitol dehydrogenase activity (e.g., lens, retina, kidneys, Schwann cells) accumulate sorbitol. Excess sorbitol causes osmotic damage and explains changes seen in hyperglycemic diabetic patients such as diabetic cataracts, diabetic retinopathy, diabetic nephropathy, and diabetic neuropathy.

Disorders of fructose metabolism

last updated 08/05/2018
{{uncollapseSections(['4Ec3DV0', 'iEcJvV0', 'QEcuvV0', 'jEc_vV0', 'PEcWDV0'])}}